DSCAM anticorps (Internal Region)

N° du produit ABIN185687

L’anticorps Chèvre Polyclonal anti-DSCAM a été validé pour ELISA, WB, FACS et IF. Il convient pour détecter DSCAM dans des échantillons de Humain.

Aperçu rapide pour DSCAM anticorps (Internal Region) (ABIN185687)

Antigène: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DSCAM est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-DSCAM anticorps

Épitope: Internal Region

Fonction: DSCAM

Séquence: DSWDSAQRTK DVSPQ

Specificité: This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DSWDSAQRTKDVSPQ, from the internal region of the protein sequence according to NP_001380.2, NP_996770.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 200-220 kDa band observed in HepG2 cell lysates (calculated MW of 174 kDa according to NP_996770.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur DSCAM

Antigène: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Autre désignation: DSCAM

Sujet: DSCAM, Down syndrome cell adhesion molecule , CHD2-42, CHD2-52 , human CHD2-52 down syndrome cell adhesion molecule

ID gène: 1826, 13508, 171119

NCBI Accession: NP_001380, NP_001258463

Pathways: Regulation of Cell Size