SECISBP2 anticorps (Internal Region)

N° du produit ABIN185714

L’anticorps Chèvre Polyclonal anti-SECISBP2 a été validé pour WB, ELISA, IF, IHC, IP et ICC. Il convient pour détecter SECISBP2 dans des échantillons de Humain et Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour SECISBP2 anticorps (Internal Region) (ABIN185714)

Antigène: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SECISBP2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Classe de qualité: Verified

Détail du produit anti-SECISBP2 anticorps

Épitope: Internal Region

Fonction: SBP2

Séquence: KERQERKQRL QEN

Réactivité croisée: Boeuf (Vache), Chien, Humain, Porc

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-KERQERKQRLQEN

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3 μg/mL.

Western Blot: Approx 120 kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 J

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec,36(22):7192-206, PMID: 19004874.
Immunoprec

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-SECISBP2 (ABIN185714)

Raman, Pitts, Seyedali, Hashimoto, Bellinger, Berry: "Selenoprotein W expression and regulation in mouse brain and neurons." dans: Brain and behavior, Vol. 3, Issue 5, pp. 562-74, (2014) (PubMed).

Papp, Wang, Kennedy, Boucher, Zhang, Gladyshev, Singh, Khanna: "Functional characterization of alternatively spliced human SECISBP2 transcript variants." dans: Nucleic acids research, Vol. 36, Issue 22, pp. 7192-206, (2008) (PubMed).

Détails sur SECISBP2

Antigène: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Autre désignation: SECISBP2

Sujet: SBP2 , RP11-89K14.1, SECISBP2, SECIS binding protein 2 , DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2

ID gène: 79048

NCBI Accession: NP_076982