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Lipotropin, beta - (AA 179-267) anticorps

Reactivité: Humain WB, ELISA, IHC (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1858144
  • Antigène
    Lipotropin, beta -
    Épitope
    AA 179-267
    Reactivité
    Humain
    Hôte
    • 3
    Lapin
    Clonalité
    • 3
    Polyclonal
    Conjugué
    • 1
    • 1
    • 1
    Inconjugué
    Application
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Séquence
    MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EL TGQRLREGDG PDGPADDGAG AQADLEHSLL VAAEKKDEGP YRMEHFRWGS PPKDKRYGGF MTSEKSQTPL VTLFKNAIIK NAYKKGE
    Specificité
    It has been selected for its ability to recognize bLPH in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogène
    bLPH (AA 179-267)
    Isotype
    IgG
  • Indications d'application
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Commentaires

    Content: The quality control contains recombinant bLPH (Glu179~Glu267) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Date de péremption
    12 months
  • Antigène
    Lipotropin, beta -
    Autre désignation
    beta-Lipotropic Hormone (bLPH)
    Synonymes
    anticorps ACTH, anticorps CLIP, anticorps LPH, anticorps MSH, anticorps NPP, anticorps POC, anticorps proopiomelanocortin, anticorps POMC
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