CA12 anticorps (AA 161-318)

N° du produit ABIN1858202

L’anticorps Lapin Polyclonal anti-CA12 a été validé pour WB, IHC, IP et ICC et a été validé indépendamment. Il convient pour détecter CA12 dans des échantillons de Souris.

Aperçu rapide pour CA12 anticorps (AA 161-318) (ABIN1858202)

Antigène: CA12 (Carbonic Anhydrase 12 (CA12))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA12 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-CA12 anticorps

Épitope: AA 161-318

Fonction: Polyclonal Antibody to Carbonic Anhydrase XII (CA12)

Specificité: The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.

Réactivité croisée: Humain, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogène: Recombinant Carbonic Anhydrase XII (CA12)

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Commentaires:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #100909 (Immunohistochemistry)

by: School of Clinical Sciences, University of Bristol

No.: #100909

Date: 21.02.2017

Antigène: CA12

Numéro du lot: A20170109527

Application validée: Immunohistochemistry

Contrôle positif: mouse kidney, mouse ciliary body

Contrôle négative: secondary antibody only

Validation Images

Immunostaining of murine kidney tissue (A.) and ciliary body sections (B.) with the CA12 antibody ABIN3204699 (green; counterstain with DAPI, blue) as described in the protocol. No staining of the plasma membrane is observed in ciliary body section stains with the secondary antibody alone (C.).

Protocole

Anticorps primaire: ABIN3204699

Anticorps secondaire: goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)

Full Protocol:

• Cryo-section:
• • Wash mouse eye and kidney tissue in PBS.
  • Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
  • Gently freeze suspended tissue in liquid nitrogen for 5min.
  • Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
  • Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
  • Rinse sections in PBS 3 times for 5min.
  • Block sections with 5% normal goat serum for 1h at RT.
• Immunostaining:
• • Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
  • Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
  • Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
  • Counterstain with DAPI in the mounting medium step.
• Wash and mount:
• • Rinse sections in PBS 3 times for 5min.
  • Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
• Microscopy:
• • Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Date de péremption: 24 months

Détails sur CA12

Antigène: CA12 (Carbonic Anhydrase 12 (CA12))

Autre désignation: CA12

Sujet: CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3