MUC16 anticorps (AA 21622-21762) (Biotin)

N° du produit ABIN1858204

Détail du produit anti-MUC16 anticorps (Biotin)

Antigène: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Épitope: AA 21622-21762

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MUC16 est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Séquence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- RVIALRSVK NGAETRVDLL CTYLQPLSGP GLPIKQVFHE LSQQTHGITR LGPYSLDKDS LYLNGYNEPG LDEPPTTPKP ATTFLPPLSE ATTAMGYHLK TLTLNFTISN LQYSPDMGKG SATFNSTEGV LQHLLRPLFQ KS

Specificité: It has been selected for its ability to recognize CA125 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant CA125 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CA125 conjugated to biotin.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant CA125 (Arg21622~Ser21762) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur MUC16

Antigène: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Autre désignation: Carbohydrate Antigen 125 (CA125) (CA125 Produits)

Synonymes: anticorps CA125, anticorps 1110008I14Rik, anticorps Ca125, anticorps Gm1480, anticorps RGD1308422, anticorps MUC16, anticorps mucin 16, cell surface associated, anticorps mucin 16, anticorps mucin-16, anticorps MUC16, anticorps Muc16, anticorps LOC476718, anticorps LOC712735