PARP4 anticorps

N° du produit ABIN187331

L’anticorps anti-PARP4 Monoclonal Souris est utilisé pour la détection de PARP4 dans des échantillons de Humain. Il a été validé pour WB et IHC (fro).

Aperçu rapide pour PARP4 anticorps (ABIN187331)

Antigène: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PARP4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: P193-6

Détail du produit anti-PARP4 anticorps

Specificité: This clone (P193-6) reacts with an internal epitope (amino acids 593-599, FSKVEDY) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor lines, accordingly to an increase in the number of vault particles.

Immunogène: Clone p193-6 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.

Isotype: IgG2b

Information d'application

Indications d'application: Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. Flow Cytometry: Optimal conditions have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: This antibody contains approximately 250ug of immunoglobulin per ml. The solution contains 1% BSA and 0.1% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur PARP4

Antigène: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Autre désignation: PARP4

Sujet: VPARP is an antibody to the minor vault protein. It reacts with internal epitope Amino Acids 593-599, FSKVEDY, of the minor vault protein.