pan Keratin anticorps

N° du produit ABIN187832

L’anticorps Lapin Polyclonal anti-pan Keratin a été validé pour IHC (p) et IHC (fro). Il convient pour détecter pan Keratin dans des échantillons de Humain, Rat, Souris, Porc, Chien, Lapin, Hamster et Avian.

Aperçu rapide pour pan Keratin anticorps (ABIN187832)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris, Porc, Chien, Lapin, Hamster, Avian

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp pan Keratin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-pan Keratin anticorps

Specificité: Recognizes keratin Species Reactivity: Most mammals and birds.

Immunogène: Keratin extracted from human skin. Antigen Location: Cytoplasm

Information d'application

Indications d'application: Immunohistochemistry - Frozen sections: Fixation for 3 min. with 1% formaldehyde is recommended. Formaldehyde-fixed, paraffin-embedded tissue sections: Proteolytic pretreatment with pronase or trypsine is recommended. Use at a 1:50 dilution in PBS or Tris. Recommended positive control: Squamous cell carcinoma, skin, cervix. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.25 mL affinity chromatography purified liquid antiserum.

Stock: 4 °C

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Keratin

Sujet: This polyclonal antibody reacts with keratin of most mammals and birds. A positive reaction is obtained with all stratified squamous epithelia and in epidermal appendages such as hairfollicles and sebaceous glands. Also the epithelia of the urinary tract, stomach, small and large intestine, uterine endometrium, bile ducts, prostate, kidney collecting tubules, thymic reticulum cells, mesothelium and to a lesser extent hepatocytes, adenocarcinomas, transitional cell carcinomas and anaplastic carcinomas can be identified by this antiserum. Renal cell adenocarcinomas and small cell anaplastic lung tumors may remain negative when examined in paraffin sections. Hepatocytes and pancreatic acini show a positive staining. Nearly all cultured epithelial cells are positive, all non-epithelial cells are negative.