ATG4B anticorps (C-Term)

N° du produit ABIN1882065

Cité dans 6 publications. Cet anticorps anti-ATG4B Polyclonal Lapin (Clone RB7560) (ABIN1882065) détecte spécifiquement ATG4B dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour ATG4B anticorps (C-Term) (ABIN1882065)

Antigène: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG4B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7560

Détail du produit anti-ATG4B anticorps

Épitope: AA 358-390, C-Term

Homologie: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 6 months

Références pour anticorps anti-ATG4B (ABIN1882065)

Li, Chen, Ye, Vogt, Yin: "A high-throughput FRET-based assay for determination of Atg4 activity." dans: Autophagy, Vol. 8, Issue 3, pp. 401-12, (2012) (PubMed).

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." dans: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, (2011) (PubMed).

Li, Hou, Wang, Chen, Shao, Yin: "Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates." dans: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7327-38, (2011) (PubMed).

Geng, Kohli, Klocke, Roth: "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent." dans: Neuro-oncology, Vol. 12, Issue 5, pp. 473-81, (2010) (PubMed).

Pontén, Westermark: "Properties of human malignant glioma cells in vitro." dans: Medical biology, Vol. 56, Issue 4, pp. 184-93, (1978) (PubMed).

Westermark, Pontén, Hugosson: "Determinants for the establishment of permanent tissue culture lines from human gliomas." dans: Acta pathologica et microbiologica Scandinavica. Section A, Pathology, Vol. 81, Issue 6, pp. 791-805, (1974) (PubMed).

Détails sur ATG4B

Antigène: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Autre désignation: ATG4B

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Poids moléculaire: 44294

NCBI Accession: NP_037457, NP_847896

UniProt: Q9Y4P1

Pathways: Autophagy