ATG4A anticorps (AA 82-111)

N° du produit ABIN1882158

Cité dans 3 publications. Cet anticorps anti-ATG4A Polyclonal Lapin (Clone RB7553) (ABIN1882158) détecte spécifiquement ATG4A dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour ATG4A anticorps (AA 82-111) (ABIN1882158)

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG4A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7553

Détail du produit anti-ATG4A anticorps

Épitope: AA 82-111

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This ATG4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from human ATG4A.

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 6 months

Références pour anticorps anti-ATG4A (ABIN1882158)

Guo, Zhou, Gao, Zhang, Wei, Hong, Chu, Duan, Zhang, Xu: "MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells." dans: PLoS ONE, Vol. 12, Issue 6, pp. e0179772, (2017) (PubMed).

Li, Hou, Wang, Chen, Shao, Yin: "Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates." dans: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7327-38, (2011) (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." dans: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, (2004) (PubMed).

Détails sur ATG4A

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Autre désignation: ATG4A

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.

Poids moléculaire: 45378

NCBI Accession: NP_443168, NP_840054

UniProt: Q8WYN0

Pathways: Autophagy