This antibody detects lead in the ppb range. Indirect ELISA endpoint titer is >1: 100,000.
Purification
Whole Antiserum
Immunogène
Lead conjugated to keyhole limpet hemocyanin (KLH) by proprietary chemistry.
Isotype
IgM
Indications d'application
Indirect ELISA: optimized for lead-RSA (rabbit serum albumin) on solid phase at 200 ng/well. Ascites diluted 1:100,000 produces an O.D. signal of 0.5. Competition ELISA: with lead-RSA on the solid phase at 200 ng/well, 50 % inhibition of antibody binding is achieved with Pb2+ at 800 ng/well. These are recommended concentrations. End users should determine optimal concentrations for their applications.Not all applications have been investigated.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Dilute in PBS or medium that is identical to that used in the assay system.
Concentration
Lot specific
Buffer
Whole antiserum
Stock
-20 °C
Stockage commentaire
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Antigène
Lead
Classe de substances
Chemical
Sujet
Exposure to potentially neurotoxic levels of lead occurs in approximately 9 % of American children under 6 years of age, and many urban dwellings still contain dangerous levels of lead paint.