PRTN3 anticorps

N° du produit ABIN190718

L’anticorps anti-PRTN3 Polyclonal Lapin est utilisé pour la détection de PRTN3 dans des échantillons de Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato et Vitis vinifera. Il a été validé pour WB, IL et Co-IP.

Aperçu rapide pour PRTN3 anticorps (ABIN190718)

Antigène: PRTN3 (Proteinase 3 (PRTN3))

Reactivité: Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato, Vitis vinifera

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRTN3 est non-conjugé

Application: Western Blotting (WB), Immunolocalization (IL), Co-Immunoprecipitation ( Co-IP)

Détail du produit anti-PRTN3 anticorps

Fonction: Anti-PR-3 / CHN | Class I chitinase

Specificité: Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

Réactivité croisée (Details): Agostis stolonifera cv. 'Penncross', Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Cicer arietinum

Homologie: Arabidopsis thaliana,Manihot esculenta,Zea mays

Purification: Protein G purified

Immunogène: Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al.

Isotype: IgG

Information d'application

Indications d'application: 8 μg/mL (WB)

Commentaires:

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détails sur PRTN3

Antigène: PRTN3 (Proteinase 3 (PRTN3))

Autre désignation: PR-3

Sujet:

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Additional information: Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Poids moléculaire: 35, 34 | 32 and 34 kDa

ID gène: 107759005

UniProt: P08252, P24091