Rubisco anticorps

N° du produit ABIN190723

Cet anticorps Lapin Polyclonal détecte spécifiquement Rubisco dans WB. Il présente une réactivité envers Arabidopsis thaliana, Spinach, Chlamydomonas, Potato, Soybean et Synechocystis PCC 6803 et a été mentionné dans 2+ publications.

Aperçu rapide pour Rubisco anticorps (ABIN190723)

Antigène: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reactivité: Arabidopsis thaliana, Spinach, Chlamydomonas, Potato, Soybean, Synechocystis PCC 6803

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rubisco est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Rubisco anticorps

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 53-55 / 53-55 kDa

Purification: serum

Immunogène: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

Information d'application

Indications d'application: Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard ECL(WB).

Commentaires:

RbcS subunit is not detected by this antibody

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-Rubisco (ABIN190723)

Kissen, Hyldbakk, Wang, Sørmo, Rossiter, Bones: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." dans: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).

Li, Zhu, Zeng, Zhang, Ye, Ou, Rehman, Heuer, Chen: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." dans: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).

Détails sur Rubisco

Antigène: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Autre désignation: Rubisco

Sujet: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Poids moléculaire: expected: 53-55 kDa, apparent: 53-55 kDa

UniProt: Q43832, P00875