Rubisco anticorps

N° du produit ABIN190723

Cet anticorps Lapin Polyclonal détecte spécifiquement Rubisco dans WB et IL. Il présente une réactivité avec des échantillons de Arabidopsis thaliana, Aucuba japonica, Orge, Blue-Green Algae (Fremyella), Manihot esculenta, Oryza sativa, Pea, Physcomytrium Patens, Poplar (Populus), Potato, Salsola laricifolia, Soybean, Spinach, Synechococcus sp., Synechocystis, Zea mays et Anabaena variabilis. Il a été cité dans 2+ publications.

Aperçu rapide pour Rubisco anticorps (ABIN190723)

Antigène: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reactivité: Arabidopsis thaliana, Aucuba japonica, Orge, Blue-Green Algae (Fremyella), Manihot esculenta, Oryza sativa, Pea, Physcomytrium Patens, Poplar (Populus), Potato, Salsola laricifolia, Soybean, Spinach, Synechococcus sp., Synechocystis, Zea mays, Anabaena variabilis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rubisco est non-conjugé

Application: Western Blotting (WB), Immunolocalization (IL)

Détail du produit anti-Rubisco anticorps

Fonction: Anti-Rubisco | 557 kDa hexadecamer

Réactivité croisée (Details): Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Not reactive in: Chlamydomonas reinhardtii (immulolocalization)

Homologie: higher plants,algae,Anthoceros agrestis,Nannochloropsis sp.

Purification: Serum

Immunogène: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

Information d'application

Indications d'application: 1 : 10 000-1 : 20 000 on 0,5-10 μg total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)

Commentaires:

RbcS subunit is not detected by this antibody

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-Rubisco (ABIN190723)

Kissen, Hyldbakk, Wang, Sørmo, Rossiter, Bones: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." dans: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).

Li, Zhu, Zeng, Zhang, Ye, Ou, Rehman, Heuer, Chen: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." dans: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).

Détails sur Rubisco

Antigène: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Autre désignation: Rubisco

Sujet: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Poids moléculaire: 53-55 | 53-55 kDa

ID gène: 2715621

UniProt: P00875, Q43832