Superoxide dismutase copper chaperone anticorps (cleaved)

N° du produit ABIN190735

Cet anticorps Lapin Polyclonal détecte spécifiquement Superoxide dismutase copper chaperone dans WB. Il présente une réactivité envers Arabidopsis thaliana, Mustard, Oryza sativa et Zea mays.

Aperçu rapide pour Superoxide dismutase copper chaperone anticorps (cleaved) (ABIN190735)

Antigène: Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))

Reactivité: Arabidopsis thaliana, Mustard, Oryza sativa, Zea mays

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Superoxide dismutase copper chaperone est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Superoxide dismutase copper chaperone anticorps

Épitope: cleaved

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including: Glycine max, Solanum tuberosum

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 33.8 / 37 kDa (Arabidopsis thaliana)

Purification: serum

Immunogène: The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Information d'application

Indications d'application: 1 : 1000 with alkaline phosphatase (WB)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Détails sur Superoxide dismutase copper chaperone

Antigène: Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))

Autre désignation: CCS

Sujet: AGI Code: At1g12520
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

Poids moléculaire: expected: 33.8 kDa, apparent: 37 kDa (Arabidopsis thaliana)

UniProt: Q4ZJI5

Pathways: Transition Metal Ion Homeostasis