Nitrogenase anticorps

N° du produit ABIN190742

Il existe 2+ publications pour ce produit. L’anticorps anti-Nitrogenase Polyclonal Poulet est utilisé pour la détection de Nitrogenase dans des échantillons de Anabaena variabilis, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii, Dolichospermum, Nostoc, Rhodopseudomonas palustris, Trichodesmium, Trifolium repens et Vibrio sp.. Il a été validé pour IF, IHC et WB.

Aperçu rapide pour Nitrogenase anticorps (ABIN190742)

Antigène: Nitrogenase (Nifh)

Reactivité: Anabaena variabilis, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii, Dolichospermum, Nostoc, Rhodopseudomonas palustris, Trichodesmium, Trifolium repens, Vibrio sp.

Hôte: Poulet

Clonalité: Polyclonal

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit anti-Nitrogenase anticorps

Fonction: Anti-NifH | Nitrogenase iron protein

Réactivité croisée (Details): Anabaena PCC7120, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii CS-505, Dolichospermum sp., Nostoc sp, Rhodopseudomonas palustris, Trichodesmium sp., nodules of Trifolium repens L., Vibrio natriegens ATCC 14048
Not reactive in: Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 as NifH protein is not present in those cyanobacterial species, Frankia sp.

Homologie: Azotobacter vinelandii (Gram-),Bradyrhizobium japonicum,Cyanobacteria,Cyanothece ATCC51142,Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum,Enterobacter,genera,euryachaeotes,Klebsiella pneumonia,Magnetococcus sp.,Methanobacterium thermoautotrophicum,Methanococcus maripaludis,Methylobacterium sp.,Mesoorhizobium loti,Rhodopseudomonas palustris TIE-1 strain,alpha,gamma,beta proteobacteria,enterobacteria,low GC gram+,high GC gram +,able to fix atmoshperic nitrogen,Rhizobium meliloti

Purification: Immunogen affinity purified

Immunogène: KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78

Information d'application

Indications d'application: 1 : 500 (IHC), 6 μg/mL (IF), 1 : 2000 (WB)

Commentaires:

This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. The anti-NifH antibody will not show a strong reactivity to this target.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.36 µg/µL

Buffer: Immunogen affinity purified IgY in PBS pH 8 and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: 4 °C

Stockage commentaire: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-Nitrogenase (ABIN190742)

Halimatul, Ehira, Awai: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." dans: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).

Levitan, Brown, Sudhaus, Campbell, LaRoche, Berman-Frank: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." dans: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).

Détails sur Nitrogenase

Antigène: Nitrogenase (Nifh)

Sujet: Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.

Poids moléculaire: 27 | 32.5 kDa

UniProt: Q2JP78, P26254, P33178, Q51296