PsbP anticorps

N° du produit ABIN190744

Cet anticorps Lapin Polyclonal détecte spécifiquement PsbP dans WB. Il présente une réactivité avec des échantillons de Arabidopsis thaliana, Orge, Chlamydomonas reinhardtii, Pinus banksiana, Spinach et Triticum aestivum. Il a été cité dans 2+ publications.

Aperçu rapide pour PsbP anticorps (ABIN190744)

Antigène: PsbP

Reactivité: Arabidopsis thaliana, Orge, Chlamydomonas reinhardtii, Pinus banksiana, Spinach, Triticum aestivum

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Détail du produit

Fonction: Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

Réactivité croisée (Details): Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Not reactive in: Synechococcus sp. PCC 7942

Homologie: Musa acuminata,Oryza sativa,Pinus monticola,Pisum sativum,Populus balsamifera,Solanum lycopersicum

Purification: Protein G purified

Immunogène: native

Information d'application

Indications d'application: 1 : 2000-1 : 5000 (WB)

Commentaires:

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Référence

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." dans: Journal of plant physiology, (2011) (PubMed).

Wang, Gu, Colby, Shindo, Balamurugan, Waldmann, Kaiser, van der Hoorn: "Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana." dans: Nature chemical biology, Vol. 4, Issue 9, pp. 557-63, (2008) (PubMed).

Détail du antigène

Antigène: PsbP

Sujet: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33- kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Poids moléculaire: 28 | 23 kDa

UniProt: P12302