GABPB2 anticorps (Internal Region)

N° du produit ABIN190778

L’anticorps Chèvre Polyclonal anti-GABPB2 a été validé pour WB, ELISA, IHC, IF et FACS. Il convient pour détecter GABPB2 dans des échantillons de Humain.

Aperçu rapide pour GABPB2 anticorps (Internal Region) (ABIN190778)

Antigène: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GABPB2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-GABPB2 anticorps

Épitope: Internal Region

Fonction: GABPB2

Séquence: SSENSSKATD ETG

Réactivité croisée: Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2, NP_002032.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 45+38 kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5 kDa according to NP_005245.2, NP_001307844.1 and of 38.1 kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GABPB2

Antigène: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Autre désignation: GABPB2

Sujet: GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53 kDa, GA binding protein transcription factor, beta subuni

ID gène: 2553

NCBI Accession: NP_005245, NP_002032, NP_001307844