TIA1 anticorps (Internal Region)

N° du produit ABIN190808

L’anticorps Chèvre Polyclonal anti-TIA1 a été validé pour WB, IF, IHC et ELISA. Il convient pour détecter TIA1 dans des échantillons de Humain.

Aperçu rapide pour TIA1 anticorps (Internal Region) (ABIN190808)

Antigène: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TIA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-TIA1 anticorps

Épitope: Internal Region

Fonction: TIA1

Séquence: PKSTYESNTK Q

Specificité: This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1, NP_071505.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band at 80 kDa and a very strong 26 kDa is a

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur TIA1

Antigène: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Autre désignation: TIA1

Sujet: TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)

ID gène: 7072, 21841, 312510

NCBI Accession: NP_071320, NP_071505