ASS1 anticorps (Internal Region)

N° du produit ABIN190834

Cet anticorps Chèvre Polyclonal détecte spécifiquement ASS1 dans WB, ELISA, IHC, FACS et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour ASS1 anticorps (Internal Region) (ABIN190834)

Antigène: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ASS1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ASS1 anticorps

Épitope: Internal Region

Fonction: Argininosuccinate synthetase 1

Séquence: ENPKNQAPPG LYTKTQD

Specificité: The variants represent identical protein (NP_000041.2 and NP_446464.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-ENPKNQAPPGLYTKTQD

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-ASS1 (ABIN190834)

Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." dans: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).

Détails sur ASS1

Antigène: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Autre désignation: ASS1

Sujet: Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase

ID gène: 445, 11898, 25698

NCBI Accession: NP_000041, NP_446464

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin