Nanog anticorps (C-Term)

N° du produit ABIN190835

L’anticorps Chèvre Polyclonal anti-Nanog a été validé pour WB, ELISA et IHC. Il convient pour détecter Nanog dans des échantillons de Humain.

Aperçu rapide pour Nanog anticorps (C-Term) (ABIN190835)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-Nanog anticorps

Épitope: C-Term

Fonction: NANOG (C Terminus)

Séquence: DLFLNYSMNM QPED

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 35 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/mL. An additional band of approx. 37 kDa is observed and is consistent with the obser

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: NANOG

Sujet: NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

ID gène: 79923

NCBI Accession: NP_079141

Pathways: Stem Cell Maintenance