DGAT1 anticorps (Internal Region)

N° du produit ABIN190886

L’anticorps Chèvre Polyclonal anti-DGAT1 a été validé pour WB, ELISA, IHC, FACS et IF. Il convient pour détecter DGAT1 dans des échantillons de Humain et Souris.

Aperçu rapide pour DGAT1 anticorps (Internal Region) (ABIN190886)

Antigène: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DGAT1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-DGAT1 anticorps

Épitope: Internal Region

Fonction: DGAT1

Séquence: QNSMKPFKDM DYS

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QNSMKPFKDMDYS, from the internal region of the protein sequence according to NP_036211.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 55 kDa band observed in Mouse Liver lysates (calculated MW of 56.8 kDa according to NP_034176.1). Recommended concentration: 0.01-0.1.0 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommende

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur DGAT1

Antigène: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Autre désignation: DGAT1

Sujet: DGAT1, diacylglycerol O-acyltransferase homolog 1 (mouse) , ARGP1, DGAT , ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase 1, diglyceride acyltransferase

ID gène: 8694, 13350, 84497

NCBI Accession: NP_036211

Pathways: Hormone Transport