CCKAR anticorps (Internal Region)

N° du produit ABIN190898

L’anticorps Chèvre Polyclonal anti-CCKAR a été validé pour WB et ELISA. Il convient pour détecter CCKAR dans des échantillons de Rat. Il y a 2+ publications disponibles.

Aperçu rapide pour CCKAR anticorps (Internal Region) (ABIN190898)

Antigène: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CCKAR est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-CCKAR anticorps

Épitope: Internal Region

Fonction: CCKA Receptor (mouse)

Séquence: KFDASQKKSA KEKR

Réactivité croisée: Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-KFDASQKKSAKEKR, from the internal region of the protein sequence according to NP_033957.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 75 kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009,2009:875167, PMID: 20011057.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-CCKAR (ABIN190898)

El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." dans: Pancreas, Vol. 39, Issue 2, pp. 127-34, (2010) (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." dans: International journal of endocrinology, Vol. 2009, pp. 875167, (2009) (PubMed).

Détails sur CCKAR

Antigène: CCKAR (Cholecystokinin A Receptor (CCKAR))

Autre désignation: Cckar

Sujet: Cckar, cholecystokinin A receptor , AW106902

ID gène: 12425, 24889

NCBI Accession: NP_033957

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour