SOD1 anticorps (Internal Region)

N° du produit ABIN190907

L’anticorps Chèvre Polyclonal anti-SOD1 a été validé pour WB, ELISA et IF. Il convient pour détecter SOD1 dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour SOD1 anticorps (Internal Region) (ABIN190907)

Antigène: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-SOD1 anticorps

Épitope: Internal Region

Fonction: SOD1

Séquence: SRKHGGPKDE ERH

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 18 kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20 kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9 kDa according to Human NP_000445.1, Mous

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells and the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Additional validation: This antibody has been

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-SOD1 (ABIN190907)

Frazziano, Al Ghouleh, Baust, Shiva, Champion, Pagano: "Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4." dans: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 2, pp. H197-205, (2014) (PubMed).

Détails sur SOD1

Antigène: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Autre désignation: SOD1

Sujet: SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) , HGNC:11179, ALS, ALS1, IPOA, SOD , Cu /Zn superoxide dismutase, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase (aa 120-154), superoxide

ID gène: 6647, 20655, 24786

NCBI Accession: NP_000445

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis