PIWIL1 anticorps (Internal Region)
Aperçu rapide pour PIWIL1 anticorps (Internal Region) (ABIN190916)
Antigène
Voir toutes PIWIL1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- Internal Region
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Fonction
- PIWI / HIWI
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Séquence
- SNRKDKYDAI KKY
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Specificité
- This antibody is expected to recognise both reported isoforms (NP_004755.2, NP_001177900.1).
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Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Porc, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2, NP_001177900.1.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
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Commentaires
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- PIWIL1 (Piwi-Like 1 (PIWIL1))
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Autre désignation
- PIWIL1
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Sujet
- PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi, MIWI, piwi homolog, piwi-like 1
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ID gène
- 9271, 57749
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NCBI Accession
- NP_004755, NP_001177900
Antigène
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