GLB1 anticorps (HRP)

N° du produit ABIN1952606

L’anticorps Lapin Polyclonal anti-GLB1 a été validé pour WB, IHC, ELISA, IP, IF et DB. Il convient pour détecter GLB1 dans des échantillons de E. coli.

Aperçu rapide pour GLB1 anticorps (HRP) (ABIN1952606)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLB1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB)

Détail du produit anti-GLB1 anticorps (HRP)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, and anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Purification: Ion exchange chromatography

Immunogène: Beta Galactosidase (E. coli).

Type of Immunogen: Purified protein

Information d'application

Indications d'application: Approved: DB, ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:2000), IP, WB (1:2000 - 1:5000)

Usage: Anti-Beta Galactosidase is suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 mu m) to produce free-floating sections. A 1:5000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1:10000 dilution was noted to show no background and to be suitable for double labeling experiments. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: E. coli

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Détails sur GLB1

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Beta Galactosidase

Pathways: Glycosaminoglycan Metabolic Process