Caveolin-1 anticorps (pTyr14)

N° du produit ABIN196778

L’anticorps anti-Caveolin-1 Polyclonal Lapin est utilisé pour la détection de Caveolin-1 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB et IF.

Aperçu rapide pour Caveolin-1 anticorps (pTyr14) (ABIN196778)

Antigène: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caveolin-1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-Caveolin-1 anticorps

Épitope: pTyr14

Specificité: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Antibody AP02388PU detects endogenous levels of Caveolin-1 only when phosphorylated at Tyrosine 14.

Purification: Immunoaffinity Chromatography.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human Caveolin-1 around the phosphorylation site of tyrosine 14 (H-L-YP-T-V).

Information d'application

Indications d'application: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur Caveolin-1

Antigène: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Autre désignation: Caveolin-1

Sujet: Caveolae (also known as plasmalemmal vesicles) are 50-100 nm flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.Synonyms: CAV1

ID gène: 857

NCBI Accession: NP_001744

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals