NCOA1 anticorps (pTyr529)
Aperçu rapide pour NCOA1 anticorps (pTyr529) (ABIN196876)
Antigène
Voir toutes NCOA1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pTyr529
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Specificité
- The antibody detects endogenous levels of Src only when phosphorylated at Tyrosine 529.
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Purification
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogène
- The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
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Indications d'application
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))
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Autre désignation
- SRC1
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Sujet
- Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src
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ID gène
- 6714
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NCBI Accession
- NP_005408
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UniProt
- P12931
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Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
Antigène
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