Histone H3.1 anticorps (pSer10)

N° du produit ABIN196922

L’anticorps anti-Histone H3.1 Polyclonal Lapin est utilisé pour la détection de Histone H3.1 dans des échantillons de Humain. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour Histone H3.1 anticorps (pSer10) (ABIN196922)

Antigène: Histone H3.1 (HIST1H3B)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H3.1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Histone H3.1 anticorps

Épitope: pSer10

Specificité: Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-Sp-T-G).

Information d'application

Indications d'application: Suitable for use in Suitable for use in Western blot (1: 500-1: 1000) andImmunohistochemistry on paraffin-embedded sections (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur Histone H3.1

Antigène: Histone H3.1 (HIST1H3B)

Autre désignation: Histone H3.1

Sujet: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.Synonyms: H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H3FA, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

ID gène: 8350

NCBI Accession: NP_003520

UniProt: P68431