Merlin anticorps (pSer518)

N° du produit ABIN197028

Cet anticorps anti-Merlin Polyclonal Lapin (ABIN197028) détecte spécifiquement Merlin dans WB et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Merlin anticorps (pSer518) (ABIN197028)

Antigène: Merlin (NF2) (Neurofibromin 2 (NF2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Merlin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-Merlin anticorps

Épitope: pSer518

Specificité: AP02513PU antibody detects endogenous levels of Merlin only when phosphorylated at Serine 518.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human Merlin around the phosphorylation site of serine 518 (R-L-SP-M-E).

Information d'application

Indications d'application: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur Merlin

Antigène: Merlin (NF2) (Neurofibromin 2 (NF2))

Autre désignation: Merlin

Sujet: Neurofibromin 2 (NF2) is a tumor suppressor gene encoding the protein Merlin. Merlin is closely related to the ERM (ezrin, radixin, moesin) family of proteins. The precise funtion of Merlin is not clear. It is thought to provide a link between the actin cytoskeleten and membrane associated proteins, playing a role in transduction of extracellular signals. It has been implicated in cell proliferation and cellular motility. Mutations in the NF2 gene cause neurofibromatosis type II, a condition characterised by the development of tumors in the central nervous system.Synonyms: Moesin-ezrin-radixin-like protein, NF2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomerlin, Schwannomin, Schwannomin

ID gène: 4771

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization