APP anticorps (AA 666-670)

N° du produit ABIN197432

Cet anticorps anti-APP Polyclonal Lapin (ABIN197432) détecte spécifiquement APP dans WB et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat. Validé indépendamment pour une utilisation en Immunofluorescence.

Aperçu rapide pour APP anticorps (AA 666-670) (ABIN197432)

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-APP anticorps

Épitope: AA 666-670

Specificité: This antibody detects endogenous levels of total APP protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Immunogène: peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.

Information d'application

Indications d'application: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Independent Validation (IF)

Validation #029576 (Immunofluorescence)

by: Molecular Pathology Core

No.: #029576

Date: 11.01.2014

Numéro du lot: 8715

Application validée: Immunofluorescence

Contrôle positif: Human brain

Contrôle négative: Human liver

Conclusion: Signal was detected in positive control tissue, and no signal was seen in negative control tissue.

Validation Images

Figure 1. Micrograph image of positive control (human brain FFPE tissue). APP staining appears in green.

Figure 2: micrograph image of negative control (human liver FFPE tissue) stained with APP antibody.

Figure 3: micrograph image of isotype control (rabbit IgG isotype control antibody on human brain FFPE tissue).

Figure 4: micrograph image of secondary antibody only control (no primary antibody on human brain FFPE tissue).

Protocole

Anticorps primaire:

• Antibody: Amyloid beta A4 precursor protein (APP)
• Catalog number: ABIN197433
• Lot number: 8715

Anticorps secondaire:

• Antibody: AlexaFluor 488 goat anti-Rabbit IgG
• Lot number: 702323

Full Protocol:

• Sections were deparaffinized and rehydrated.
• Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
• Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
• Sections were washed x 2 in 1xTBS buffer.
• Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
• Sections were washed x 2 in 1xTBS buffer.
• Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
• Sections were washed x 3 in 1xTBS buffer.
• Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
• Sections were photographed with a Zeiss Axioskop2 microscope

Notes: None

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur APP

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Autre désignation: Amyloid beta A4 Protein / APP

Sujet: Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

ID gène: 351

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour