Selectin E/CD62e anticorps

N° du produit ABIN198855

Cet anticorps anti-Selectin E/CD62e est un anticorps Souris Monoclonal détectant Selectin E/CD62e dans IHC, IP et IHC (fro). Adapté pour Humain et Singe.

Aperçu rapide pour Selectin E/CD62e anticorps (ABIN198855)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Selectin E/CD62e anticorps

Specificité: Reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). Reacts with human endothelial cells activated with TNF-a, IL-1 or endotoxin. Found to react also with cells transfected with the E-selectin gene. Inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.

Purification: Purified

Immunogène:

Type of Immunogen: Native protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-Fr, IP

Usage: The antibody is useful for staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey and baboon endothelial cells expressing E-selectin. Furthermore the antibody can be used for Immunoprecipitation and immunoassays. Immunohistology: 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-a (1 ng/mL), IL-1 (100U/mL) or LPS (1 μg/mL) in tissue culture medium at 37 C. As negative control it is advised to use a control murine IgG1 antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: SELE / CD62E / E-selectin

Sujet: Name/Gene ID: SELE

Synonyms: SELE, CD62E, ELAM, ESEL, E-selectin, LECAM2, CD62E antigen, ELAM-1, ELAM1, Selectin E

ID gène: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling