Selectin E/CD62e anticorps
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- Antigène Voir toutes Selectin E/CD62e (SELE) Anticorps
- Selectin E/CD62e (SELE) (Selectin E (SELE))
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Reactivité
- Humain, Singe
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Selectin E/CD62e est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- Reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). Reacts with human endothelial cells activated with TNF-a, IL-1 or endotoxin. Found to react also with cells transfected with the E-selectin gene. Inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
- Purification
- Purified
- Immunogène
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Type of Immunogen: Native protein - Isotype
- IgG1
- Top Product
- Discover our top product SELE Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-Fr, IP
Usage: The antibody is useful for staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey and baboon endothelial cells expressing E-selectin. Furthermore the antibody can be used for Immunoprecipitation and immunoassays. Immunohistology: 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-a (1 ng/mL), IL-1 (100U/mL) or LPS (1 μg/mL) in tissue culture medium at 37 C. As negative control it is advised to use a control murine IgG1 antibody. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.02 % sodium azide, 0.1 % BSA.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Antigène
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- Autre désignation
- SELE / CD62E / E-selectin (SELE Produits)
- Synonymes
- anticorps SELE, anticorps CD62E, anticorps ELAM, anticorps ELAM1, anticorps ESEL, anticorps LECAM2, anticorps E-selectin, anticorps Elam, anticorps selectin E, anticorps selectin, endothelial cell, anticorps SELE, anticorps Sele
- Sujet
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Name/Gene ID: SELE
Synonyms: SELE, CD62E, ELAM, ESEL, E-selectin, LECAM2, CD62E antigen, ELAM-1, ELAM1, Selectin E - ID gène
- 6401
- UniProt
- P16581
- Pathways
- Thromboxane A2 Receptor Signaling
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