TYR anticorps (AA 1-433)

N° du produit ABIN199780

Cet anticorps Souris Monoclonal détecte spécifiquement TYR dans WB, IHC, IF, IHC (p) et ICC. Il présente une réactivité envers Humain, Chien et Cat.

Aperçu rapide pour TYR anticorps (AA 1-433) (ABIN199780)

Antigène: TYR (Tyrosinase (TYR))

Reactivité: Humain, Chien, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-TYR anticorps

Épitope: AA 1-433

Specificité: Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.

Purification: Protein A purified

Immunogène: Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ICC, IF, IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistology: 2-4 μg/mL for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur TYR

Antigène: TYR (Tyrosinase (TYR))

Autre désignation: Tyrosinase

Sujet: Name/Gene ID: TYR

Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

ID gène: 7299

UniProt: P14679