BUB1B anticorps (AA 1-350)

N° du produit ABIN199935

Cet anticorps anti-BUB1B est un anticorps Souris Monoclonal détectant BUB1B dans WB, IF et IP. Adapté pour Humain.

Aperçu rapide pour BUB1B anticorps (AA 1-350) (ABIN199935)

Antigène: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BUB1B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-BUB1B anticorps

Épitope: AA 1-350

Specificité: Amino acid residues 1-350 of human BUBR1 Kinase protein.

Purification: Protein A purified

Immunogène: Recombinant human BUB1B / BubR1.

Type of Immunogen: Recombinant protein

Information d'application

Indications d'application: Approved: IF, IP, WB

Usage: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at  pH 6.8. Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl,  pH 7.4 containing 0.1 % BSA.

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur BUB1B

Antigène: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Autre désignation: BUB1B / BubR1

Sujet: Name/Gene ID: BUB1B
Subfamily: BUB1
Family: Protein Kinase

Synonyms: BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L

ID gène: 701

UniProt: O60566