Histone H2B anticorps (acLys12, acLys15, acLys20)

N° du produit ABIN201041

L’anticorps Lapin Polyclonal anti-Histone H2B a été validé pour WB. Il convient pour détecter Histone H2B dans des échantillons de Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour Histone H2B anticorps (acLys12, acLys15, acLys20) (ABIN201041)

Antigène: Histone H2B

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Histone H2B anticorps

Épitope: acLys12, acLys15, acLys20

Specificité: Recognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species cross-reactivity: Human. Predicted to cross-react with mouse, rat and bovine based on sequence homology.

Purification: Protein A purified

Immunogène: KLH-conjugated, synthetic peptide (. S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ.) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: WB

Usage: Suitable for use in Western Blot. Western Blot Analysis: 1:40000-1:80000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Histone H2B

Antigène: Histone H2B