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Plakophilin 3 anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement Plakophilin 3 dans WB, ELISA, IHC, IP, IF et IHC (p). Il présente une réactivité envers Humain, Souris et Chien.
N° du produit ABIN201418

Aperçu rapide pour Plakophilin 3 anticorps (ABIN201418)

Antigène

Voir toutes Plakophilin 3 (PKP3) Anticorps
Plakophilin 3 (PKP3)

Reactivité

  • 31
  • 8
  • 5
  • 2
  • 1
  • 1
  • 1
Humain, Souris, Chien

Hôte

  • 16
  • 13
  • 2
Souris

Clonalité

  • 17
  • 14
Monoclonal

Conjugué

  • 26
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Plakophilin 3 est non-conjugé

Application

  • 31
  • 12
  • 11
  • 11
  • 10
  • 5
  • 5
  • 5
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Specificité

    Reacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.

    Purification

    Protein A purified

    Immunogène

    Synthetic peptide from Armadillo domain of human Plakophilin-3.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG2b
  • Indications d'application

    Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P, IP, WB (1 - 3 μg/mL)

    Usage: Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 10 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Immunofluorescence: 1-3 μg/mL. Western Blot: 1-3 μg/mL. Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2 % Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % sodium azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Antigène

    Plakophilin 3 (PKP3)

    Autre désignation

    PKP3 / Plakophilin 3

    Sujet

    Name/Gene ID: PKP3

    Synonyms: PKP3, Plakophilin 3, Plakophilin-3, Plakophilin 3b

    ID gène

    11187

    UniProt

    Q9Y446
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