IL17D anticorps (AA 18-202)
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- Antigène Voir toutes IL17D Anticorps
- IL17D (Interleukin 17D (IL17D))
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Épitope
- AA 18-202
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp IL17D est non-conjugé
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- Recognizes human IL-27. Does not cross-react with rhIL-17, rhIL17B, rhIL-17C, rhIL-17E, or rhIL-17F. Species sequence homology: Mouse: 86 % .
- Purification
- Protein G purified
- Immunogène
- E. coli-derived recombinant human Interleukin 27 (aa18-202).
- Clone
- 246018
- Isotype
- IgG2b
- Top Product
- Discover our top product IL17D Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-P, WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Western Blot: 1-2 μg/mL. Detection limit is approximately 25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Add 10 μL of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. Incubate for 30 minutes and then wash cells with 0.1 % saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1 % saponin prior to flow cytometric analysis. Direct ELISA: 0.5-1 μg/mL. Detection limit is ~5 ng/well. Immunohistochemistry: 8-25 μg/mL with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with sterile 40-50 % glycerol, PBS.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
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- Antigène
- IL17D (Interleukin 17D (IL17D))
- Autre désignation
- IL17D (IL17D Produits)
- Synonymes
- anticorps IL-17D, anticorps IL-22, anticorps IL-27, anticorps IL27, anticorps AI462269, anticorps Il27a, anticorps il17-4, anticorps zgc:194257, anticorps zgc:194303, anticorps IL-27p28, anticorps Il30, anticorps p28, anticorps interleukin 17D, anticorps interleukin 17d, anticorps interleukin 27, anticorps IL17D, anticorps Il17d, anticorps il17d, anticorps Il27
- Sujet
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Name/Gene ID: IL17D
Family: Interleukin
Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D - ID gène
- 53342
- UniProt
- Q8TAD2
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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