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Occludin anticorps (C-Term) (FITC)

L’anticorps Souris Monoclonal anti-Occludin a été validé pour WB, ELISA et IF. Il convient pour détecter Occludin dans des échantillons de Humain, Souris, Rat et Chien.
N° du produit ABIN204503

Aperçu rapide pour Occludin anticorps (C-Term) (FITC) (ABIN204503)

Antigène

Voir toutes Occludin (OCLN) Anticorps
Occludin (OCLN)

Reactivité

  • 113
  • 62
  • 44
  • 34
  • 18
  • 12
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
Humain, Souris, Rat, Chien

Hôte

  • 91
  • 28
  • 3
  • 1
Souris

Clonalité

  • 86
  • 37
Monoclonal

Conjugué

  • 57
  • 10
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Occludin est conjugé à/à la FITC

Application

  • 101
  • 34
  • 31
  • 31
  • 26
  • 26
  • 16
  • 16
  • 13
  • 12
  • 10
  • 6
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone

OC-3F10
  • Épitope

    • 16
    • 16
    • 13
    • 10
    • 9
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificité

    Recognizes human Occludin Protein. Species cross-reactivity: mouse, rat and canine.

    Purification

    Protein A purified

    Immunogène

    GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

    Type of Immunogen: Fusion protein

    Isotype

    IgG1 kappa
  • Indications d'application

    Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 10 μg/mL), WB (0.1 - 1 μg/mL)

    Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. ELISA: 0.1-1 μg/mL. Western Blot: Detects a band at ~65kD. Immunofluorescence: 2-10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect from light.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C
    Long term: -20°C
    Protect from light.
  • Antigène

    Occludin (OCLN)

    Autre désignation

    OCLN / Occludin

    Sujet

    Name/Gene ID: OCLN

    Synonyms: OCLN, Occludin, BLCPMG

    ID gène

    100506658

    UniProt

    Q16625

    Pathways

    Cell-Cell Junction Organization, Hepatitis C
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