DNMT3L anticorps (AA 152-164)

N° du produit ABIN208362

Cet anticorps anti-DNMT3L est un anticorps Lapin Polyclonal détectant DNMT3L dans WB, ELISA et IP. Adapté pour Humain.

Aperçu rapide pour DNMT3L anticorps (AA 152-164) (ABIN208362)

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT3L est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-DNMT3L anticorps

Épitope: AA 152-164

Specificité: Recognizes Human DNMT3L MW: 43.6kD. Species sequence Homology: Mouse: 76 % .

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey (92%) Rat, Hamster, Panda, Bat (85%).

Purification: Immunoaffinity purified

Immunogène: A synthetic peptide corresponding to aa 152-164, GLLQRRRKWRSQL of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey (92%), Rat, Hamster, Panda, Bat (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA (1:1000 - 1:3000), IP, WB (1:1500)

Usage: Suitable for use in ELISA, Immunoprecipitation and Western Blot. ELISA: 1:1000-1:3000. Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells. (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150  mM. Does not perform well when applied directly for Western Blot.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur DNMT3L

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Autre désignation: DNMT3L

Sujet: Name/Gene ID: DNMT3L
Family: C5-methyltransferase

Synonyms: DNMT3L

ID gène: 29947

UniProt: Q9UJW3