ZBTB33 anticorps (AA 103-584)

N° du produit ABIN210906

Cet anticorps anti-ZBTB33 est un anticorps Souris Monoclonal détectant ZBTB33 dans WB, IP et IHC. Adapté pour Humain, Souris, Rat, Chien, Poulet et Hamster.

Aperçu rapide pour ZBTB33 anticorps (AA 103-584) (ABIN210906)

Antigène: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Reactivité: Humain, Souris, Rat, Chien, Poulet, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ZBTB33 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Détail du produit anti-ZBTB33 anticorps

Épitope: AA 103-584

Specificité: Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.

Purification: Protein G purified

Immunogène: Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).

Type of Immunogen: Recombinant protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ZBTB33

Antigène: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Autre désignation: ZBTB33 / Kaiso

Sujet: Name/Gene ID: ZBTB33

Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348

ID gène: 10009

UniProt: Q86T24