ZBTB33 anticorps (AA 103-584)
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- Antigène Voir toutes ZBTB33 Anticorps
- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
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Épitope
- AA 103-584
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Reactivité
- Humain, Souris, Rat, Chien, Poulet, Hamster
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ZBTB33 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Specificité
- Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
- Purification
- Protein G purified
- Immunogène
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Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).
Type of Immunogen: Recombinant protein - Isotype
- IgG1
- Top Product
- Discover our top product ZBTB33 Anticorps primaire
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- Indications d'application
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Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex. - Commentaires
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Antigène
- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
- Autre désignation
- ZBTB33 / Kaiso (ZBTB33 Produits)
- Synonymes
- anticorps kaiso, anticorps ZNF-kaiso, anticorps ZNF348, anticorps AW260253, anticorps E130014G12Rik, anticorps Kaiso, anticorps Znf-kaiso, anticorps RGD1566309, anticorps zinc finger and BTB domain containing 33 S homeolog, anticorps zinc finger and BTB domain containing 33, anticorps zbtb33.S, anticorps ZBTB33, anticorps Zbtb33
- Sujet
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Name/Gene ID: ZBTB33
Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348 - ID gène
- 10009
- UniProt
- Q86T24
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