Histone H1 anticorps (hyperphosphorylated)

N° du produit ABIN211181

L’anticorps anti-Histone H1 Polyclonal Lapin est utilisé pour la détection de Histone H1 dans des échantillons de Humain. Il a été validé pour WB et ICC.

Aperçu rapide pour Histone H1 anticorps (hyperphosphorylated) (ABIN211181)

Antigène: Histone H1

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Détail du produit anti-Histone H1 anticorps

Épitope: hyperphosphorylated

Specificité: Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.

Purification: Protein A purified

Immunogène: Hyper-phosphorylated isoform of Tetrahymena Histone H1.

Type of Immunogen: Purified protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ICC (5 - 10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95 % ethanol, 5 % glacial acetic acid.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Histone H1

Antigène: Histone H1