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Histone H2B anticorps (AA 118-126)

Cet anticorps anti-Histone H2B est un anticorps Lapin Polyclonal détectant Histone H2B dans WB. Adapté pour Humain.
N° du produit ABIN211182

Aperçu rapide pour Histone H2B anticorps (AA 118-126) (ABIN211182)

Antigène

Voir toutes Histone H2B Anticorps
Histone H2B

Reactivité

  • 218
  • 92
  • 85
  • 12
  • 10
  • 5
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
Humain

Hôte

  • 223
  • 22
  • 2
Lapin

Clonalité

  • 213
  • 34
Polyclonal

Conjugué

  • 170
  • 12
  • 8
  • 7
  • 4
  • 4
  • 4
  • 4
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  • 2
Cet anticorp Histone H2B est non-conjugé

Application

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  • 34
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  • 21
  • 17
  • 7
  • 6
  • 6
  • 6
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB)
  • Épitope

    • 23
    • 15
    • 12
    • 11
    • 11
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    • 10
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    • 9
    • 7
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    • 1
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    • 1
    AA 118-126

    Specificité

    Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is detected in some samples, which likely corresponds to ubiquityl-Histone H2B. Species cross-reactivity: Human, chicken and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.

    Homologie

    Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).

    Purification

    Protein A purified

    Immunogène

    KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    Isotype

    IgG
  • Indications d'application

    Approved: WB (1:1000 - 1:10000)

    Usage: Suitable for use in Western Blot. Western Blot: 1:1000-1:10000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    200 μLof 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Antigène

    Histone H2B
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