CD58 anticorps (Cytoplasmic Domain)

N° du produit ABIN211812

Cet anticorps anti-CD58 est un anticorps Souris Monoclonal détectant CD58 dans FACS et WB. Adapté pour Humain.

Aperçu rapide pour CD58 anticorps (Cytoplasmic Domain) (ABIN211812)

Antigène: CD58

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD58 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB)

Détail du produit anti-CD58 anticorps

Épitope: Cytoplasmic Domain

Specificité: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on hemopoietic cells in individuals with paroxysmal nocturnal hemoglobinuria. BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. Reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect hemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.

Purification: Protein G purified

Immunogène: Human erythrocytes.

Type of Immunogen: Cells

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo (1:10), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Not recommended for: IHC-Fr, IHC-P

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur CD58

Antigène: CD58

Autre désignation: CD58

Sujet: Name/Gene ID: CD58
Family: Immunoglobulin

Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule

ID gène: 965