Vimentin anticorps

N° du produit ABIN213302

L’anticorps Souris Monoclonal anti-Vimentin a été validé pour WB, IHC, IHC (p) et ICC. Il convient pour détecter Vimentin dans des échantillons de Humain, Rat, Porc, Boeuf (Vache), Poulet, Singe, Chien et Cat.

Aperçu rapide pour Vimentin anticorps (ABIN213302)

Antigène: Vimentin (VIM)

Reactivité: Humain, Rat, Porc, Boeuf (Vache), Poulet, Singe, Chien, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-Vimentin anticorps

Specificité: Recognizes a 57-60kD protein, human Vimentin. Shows no cross-reactivity with other closely related intermediate filament proteins including desmin and GFAP. Species cross-reactivity: monkey, bovine, canine, feline, chicken and rat. Does not recognize mouse.

Purification: Purified

Immunogène: Purified vimentin from porcine eye lens.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ICC (5 - 10 μg/mL), IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 5-10 μg/mL, Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity. Immunocytochemistry: 5-10 μg/mL. Positive control: Breast carcinoma tissue, colon, prostate, tonsil, tonsilar lymphoma, uterus and ureter.

Commentaires:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis