COL2 anticorps

N° du produit ABIN2137989

L’anticorps Lapin Polyclonal anti-COL2 a été validé pour WB, IHC, ELISA et IP. Il convient pour détecter COL2 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour COL2 anticorps (ABIN2137989)

Antigène: COL2 (Collagen, Type II (COL2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Détail du produit anti-COL2 anticorps

Specificité: This lot of antisera reacts to both human and bovine PTH C-Terminal region.

Réactivité croisée: Rat (Rattus), Souris

Réactivité croisée (Details): This antibody that has been prepared bi immunoaffininty chromotography using immobilized antigens is followed by extensive cross-absorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins toremove any unwanted specifities.

Purification: Purified by affinity chromatography.

Stérilité: Sterile filtered

Immunogène: Collagen type II antibody was raised in rabbit using collagen type II from human knee cartilage and bovine nasal cartilage as the immunogen.

Information d'application

Indications d'application: ELISA: 1:5,000-1:10,000, IHC: 1:50-1,200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Commentaires:

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid In 0.125 M Na2B4O7, pH 8.0, with 0.075 M NaCl, 0.005 M EDTA, and 0.01 % Sodium azide.

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Références pour anticorps anti-COL2 (ABIN2137989)

Murphy, Huey, Hu, Athanasiou: "TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." dans: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).

Détails sur COL2

Antigène: COL2 (Collagen, Type II (COL2))

Autre désignation: Collagen Type II