NEK2 anticorps (AA 287-299)

N° du produit ABIN213864

Cet anticorps anti-NEK2 Polyclonal Lapin (ABIN213864) détecte spécifiquement NEK2 dans WB, ELISA, IHC, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour NEK2 anticorps (AA 287-299) (ABIN213864)

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NEK2 anticorps

Épitope: AA 287-299

Specificité: Aa 287-299 of Human NEK2 protein.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from human NEK2.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Détails sur NEK2

Antigène: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Autre désignation: NEK2

Sujet: Name/Gene ID: NEK2
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2

ID gène: 4751

UniProt: P51955

Pathways: M Phase