Fibronectin anticorps

N° du produit ABIN2141941

L’anticorps anti-Fibronectin Polyclonal Lapin est utilisé pour la détection de Fibronectin dans des échantillons de Humain. Il a été validé pour WB, IHC, IP et ELISA.

Aperçu rapide pour Fibronectin anticorps (ABIN2141941)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA

Détail du produit anti-Fibronectin anticorps

Specificité: This antibody is specific for Human Factor XIII.

Réactivité croisée (Details): This antibody reacts with Human Fibronectin. Ths antibody has negligible cross reactivity with Type I,II,III,IV,V,VI Collagens or Laminin1.0 mg/ml

Purification: Affinity chromatography purified

Stérilité: Sterile filtered

Immunogène: Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Information d'application

Indications d'application: ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid In 0.02 M K3PO4, pH 7.2, with 0.15 M NaCl, and 0.01 % Sodium azide.

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.