SAE1 anticorps

N° du produit ABIN214379

L’anticorps anti-SAE1 Polyclonal Lapin est utilisé pour la détection de SAE1 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IF, IHC et IHC (p).

Aperçu rapide pour SAE1 anticorps (ABIN214379)

Antigène: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SAE1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SAE1 anticorps

Specificité: Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST.

Purification: Protein A purified

Immunogène: Recombinant human SAE1.

Type of Immunogen: Recombinant protein

Information d'application

Indications d'application: Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2 with 0.01 % (w/v) Sodium Azide

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur SAE1

Antigène: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Autre désignation: SAE1

Sujet: Name/Gene ID: SAE1

Synonyms: SAE1, Activator of SUMO1, AOS1, UBLE1A, HSPC140, SUA1

ID gène: 10055

UniProt: Q9UBE0