CD9 anticorps (FITC)

N° du produit ABIN2144480

L’anticorps Souris Monoclonal anti-CD9 a été validé pour FACS et IF. Il convient pour détecter CD9 dans des échantillons de Humain.

Aperçu rapide pour CD9 anticorps (FITC) (ABIN2144480)

Antigène: CD9

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD9 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 4AHI9a

Détail du produit anti-CD9 anticorps (FITC)

Attributs du produit: 4AHI9a reacts with CD9 ( p24), a member of the tetraspan ( TM4SF ) family with 24 kDa MW, expressed on platelets and weakly on B-cells. It also expressed on eosinophils, basophils, endothelial and epithelial cells. CD9 antigen modulates cell adhesion, migration and platelet activation. GM1CD9 triggers platelet activation resulted in platelet aggregation, but it is blocked by anti-Fc receptor antibody CD32. This clone is cross reactive with non-human primate.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD9

Antigène: CD9

Autre désignation: CD9

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization