CD69 anticorps (FITC)
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- Antigène Voir toutes CD69 Anticorps
- CD69
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD69 est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS)
- Attributs du produit
- The 4AFN50 monoclonal antibody reacts with human CD69, also known as very eary activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
- Clone
- 4AFN50
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD69 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD69
- Autre désignation
- CD69 (CD69 Produits)
- Synonymes
- anticorps 5830438K24Rik, anticorps AI452015, anticorps AIM, anticorps VEA, anticorps BL-AC/P26, anticorps CLEC2C, anticorps EA1, anticorps GP32/28, anticorps MLR-3, anticorps Cd69 molecule, anticorps CD69 antigen, anticorps CD69 molecule, anticorps Cd69, anticorps CD69
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